Toxicity Evaluation and Tissue Damaging Effects of Cadmium in Labeo rohita: Toxicity and Tissue Damage of Cadmium in Labeo rohita

Syeda Alveena Hashmi; Muhammad Ahsan Raza; Ali Hasan; Sibgha Farooq; Arshia Mukhtar

doi:10.54393/mjz.v5i02.115

Authors

Syeda Alveena Hashmi Department of Zoology, University of Gujrat, Gujrat, Pakistan
Muhammad Ahsan Raza Department of Zoology, Government Graduate College (Boys), Satellite Town, Gujranwala, Pakistan
Ali Hasan Department of Honeybee Research, University of the Punjab, Lahore, Pakistan
Sibgha Farooq Department of Microbial Biotechnology, University of The Punjab, Lahore, Pakistan
Arshia Mukhtar Department of Microbial Biotechnology, University of The Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.54393/mjz.v5i02.115

Keywords:

Comet Assay, Bioaccumulation, Cadmium, Heavy Metals, Labeo rohita

Abstract

Heavy metals are polluting the freshwater ecosystems with hazardous impacts on aquatic animals especially fish. Considerable amounts of Cadmium (Cd) in aquatic ecosystems are posing a serious threat to sustainable growth of aquaculture industry. Objective: To investigate acute toxicity of Cd (96-hour LC₅₀and lethal concentrations) was determined by utilizing probit analysis method for the freshwater fish Labeo rohita. Methods: Different organs of fish (liver, gills and muscles) were also analyzed for accumulation of Cd by inductively coupled plasma mass spectrometry (ICP-MS). Results: Acute toxicity of Cd (96-hour LC₅₀) for Labeo rohita was calculated as 159.59 mg/L. Bioaccumulation of Cd in different tissues of fish was examined in the following order liver>gills> muscles. Comet assay was performed for the evaluation of DNA damage by different sub-lethal doses (1/2, 1/3, 1/4 of LC₅₀) of Cd. Conclusions: This research will aid in control of heavy metals contamination and conservation of fish species Labeo rohita in natural aquatic habitats of Pakistan.

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