Adverse Effects of Propranolol on Testis and Heart Tissue of Albino Mice: Adverse Effects of Propranolol

Javaria Shaheen; . Asmatullah; Azeem Azam; Ata Ul Mustafa Fahid; Hamza Faseeh; Farhan Anjum; Muhammad Rehan Faisal; Maryam Latif

doi:10.54393/mjz.v5i02.104

Authors

Javaria Shaheen Institute of Zoology, University of the Punjab, Lahore, Pakistan
. Asmatullah Institute of Zoology, University of the Punjab, Lahore, Pakistan
Azeem Azam Institute of Zoology, University of the Punjab, Lahore, Pakistan
Ata Ul Mustafa Fahid Institute of Zoology, University of the Punjab, Lahore, Pakistan
Hamza Faseeh Institute of Zoology, University of the Punjab, Lahore, Pakistan
Farhan Anjum Institute of Zoology, University of the Punjab, Lahore, Pakistan
Muhammad Rehan Faisal Department of Chemistry, The University of Lahore, Lahore, Pakistan
Maryam Latif Institute of Zoology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.54393/mjz.v5i02.104

Keywords:

Testis Toxicity, Albino Mice, Laminar Degeneration, Morphometric Analysis

Abstract

Propranolol is a non-selective, beta-adrenergic antagonist drug useful in various clinical disorders such as schizophrenia, anxiety, agitation, Autism Spectrum Disorder (ASD), hypertension, insomnia and other psychosis. Objective: To investigate the probable toxic effects of propranolol on the testis and heart of male mice. Methods: Twenty mature male mice were randomized and divided into four groups. The control group received distilled water, and the three experimental dose groups, D-I, D-II, and D-III, received 0.1 ml of propranolol using an insulin syringe with a butterfly needle in three different concentrations i.e. 0.15 µg/g, 0.30 µg/g, and 0.60 µg/g of their body weight, respectively, for a period of four weeks. After completion of the experiment, these mice were weighed, anaesthetized, and dissected. Their serum was collected by the intracardial puncture technique for the analysis of testosterone. Testes and heart were separated from the control and experimental groups for morphometric and histopathological studies. Results: The mice exposed to propranolol revealed a significant reduction in testosterone (P≤ 0.01) and body weight (P≤ 0.01). Histopathological findings also revealed laminar/tubular degeneration, vaccination, and an enlarged amyloid body in testicle tissues, while damaged striated strips and irregular arrangements of nuclei were observed in heart tissues. Conclusions: This investigation clearly reveals the adverse effects of propranolol on the testis and heart tissue of albino mice.

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