Frequency of Micro-organisms in Different Biological Samples of Patients in a Tertiary Care Hospital of Lahore, Pakistan: Isolation of microorganisms from biological samples

Ansar Abbas; Sidra Khalid; Syeda Uroosa  Jafri

doi:10.54393/mjz.v1i2.9

Authors

Ansar Abbas Department of Biotechnology, Virtual University, Lahore, Pakistan
Sidra Khalid Faculty of Allied Health Sciences (FAHS), The University of Lahore, Lahore, Pakistan
Syeda Uroosa Jafri Department of Food and Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan

DOI:

https://doi.org/10.54393/mjz.v1i2.9

Keywords:

Infection, Bacteria, Gram-positive, Gram-negative, samples, Antibody resistance

Abstract

Bacterial infections are one of the leading causes of illness and death across the world. In this context, the spread of resistant microbes plays a key role. Due to insufficient treatment choices and the sluggish discovery of new antibiotic classes, the rapid emergence of multidrug resistance in bacteria poses a serious threat to global public health. Objective: To determine the frequency of clinical isolates in various samples. Methods: A cross-sectional investigation was undertaken at the Pathology Department of the Fatima Memorial Hospital in Lahore, Pakistan. The study lasted one year. A total of 1,200 samples (sputum, wound swabs, blood, urine, pus, cerebrospinal fluid, and semen) were collected. Each sample was taken in a sterile container. The sample container was labeled with the source, date, and time of collection within one hour of collection and brought to the laboratory for analysis. Bacteria were identified using traditional culture and biochemical testing. Results: A total of 1200 clinical isolates were identified. Positive cultures obtained from 1200 biological samples were mostly from Urine (71.1%). 55.3% of the positive sample were male and 73% of the clinical isolates were gram-negative. Among Gram-negative isolates, the most common pathogen was Klebsiella species (42.9%) and among Gram-Positive isolates, the most common pathogen was Staphylococcus Aureus 68.5%. Conclusions: Gram-negative bacterial isolates were prevalent, with 55% frequency distribution and Staphylococcus aureus was frequent in different samples of patients.

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