Molecular Exploration of Zinc Finger BED-Type Containing 6 Gene for Growth Trait in Beetal Goat
DOI:
https://doi.org/10.54393/mjz.v2i1.32Keywords:
Zinc finger, BED-type containing 6, Chromatin immunoprecipitation (ChIP), C2C12 cellsAbstract
Zinc finger, BED-type containing 6 (ZBED6) is a newly discovered transcription factor. It functions as a repressor of IGF2 transcription in skeletal muscle myogenesis and development. It plays a role in organism development, signaling, cell-cell contact, hepatic fibrosis, clathrin-mediated endocytosis, and tight junction signaling cascades. Using C2C12 cells, chromatin immunoprecipitation (ChIP) sequencing revealed roughly 2,500 ZBED6 binding sites in the genome, with the inferred consensus motif matching the known binding site in Igf2. Igf2 expression, cell proliferation, wound healing, and myotube formation is all affected when Zbed6 is silenced in myoblast cells. Certain Gene Ontology categories, such as development and transcriptional control, were significantly enriched in genes related to ZBED6 binding sites. Objective: To explore the molecular expression of the ZBED6 gene that affect the growth trait in Beetal goat Methods: The BED6 gene's functional and signaling experiments revealed that it controls growth characteristics in goats. A total of 50 blood samples were taken. DNA was extracted using an inorganic technique. Primer3 software was used to build primers for PCR amplification. On the ABI 3130XL Genet Analyzer, PCR results were sequenced bi-directionally. Results: The CHROMAS program was used to examine the sequencing findings. SNPs were discovered using sequence alignment tools such as blast 2. The bovine ZBED6 gene was found on chromosome 16, has only one exon, and encodes 980 amino acids. The genomic DNA of both cow breeds was effectively amplified in this investigation by utilizing primer pairs for the ZBED6 gene. These animals had three SNPs, including one non-coding mutation in the promoter (SNP1: 826G > A) and two missense mutations in the CDS (SNP2: 680C > G and SNP3: 1043A > G). The missense mutations p. Ala 227 Gly and p. His 3" are found in SNP2 and 3. Conclusions: Novel variations have been discovered that might be used in the selection of superior goats with higher weight increase tendencies.
References
Hirst KK. The domestication history of goats (Capra hircus)2015.
MS Muhammad, MA, MS Khan, K Javed and M A Jabbar. Farmers preferences for goat breeds in punjab, Pakistan The Journal of Animal & Plant Sciences.25(2):2015,380-386.
Khan A. 2014. punjabi goats: (beetal): goats of pakistan /different goat types in pakistan.
Saif R, Henkel J, Jagannathan V, Drögemüller C, Flury C, Leeb T. The LCORL Locus is under Selection in Large-Sized Pakistani Goat Breeds. Genes (Basel). 2020;11(2):168. doi: 10.3390/genes11020168.
Ramzan F, Khan MS, Bhatti SA, Gültas M, Schmitt AO. Survey data to identify the selection criteria used by breeders of four strains of Pakistani beetal goats. Data Brief. 2020;32:106051. doi: 10.1016/j.dib.2020.106051.
Sajjad Khan, M., Ali, A., Hyder, A. U., and Chatta, A. I.: Effect of inbreeding on growth and reproduction traits of Beetal goats (short communication), Arch. Anim. Breed., 50: 197–203.doi.org/10.5194/aab-50-197-2007, 2007.
Magotra A,Bangar YC, Chauhan A, Malik BS, Malik ZS. Influence of maternal and addictive genetic effects on offsprings growth in beetal goat. Reproduction in domestic animals, 2021, 56 (7):983-991. doi.org/10.1111/rda.13940
Manish K, Jindal R, Nayyar S, Singla M. Physiological and biochemical responses in beetal goats during summer season. The Indian J of small ruminants, 2010, 16(2):255-257.
Maroof A, Singh PK, Sadana DK,Alam S, Chahal D. Reproductive performance of beetal goats in its breeding tract. The Indian J of small ruminants, 2007, 13 (9):182-185.
Manish K, Jindal R, Nayyar S. Influence of heat stress on antioxidant status in beetal goats, 2011, 17(2):178-181.
Hadizadeh M. Niazi A, Mohammad Abadi M, Esmailizadeh A, Mehdizadeh Gazooei Y.
Bioinformatics Analysis of the Bmp15 Exon 2 In Tali and Beetal Goats. Modern Genetics Journal, 2014, 9,1(36):117-120.
Ghazal A. Bioinformatics analysis of ZBED6: interactome of novel transcription factor ZBED6 in C2C12 myoblasts. 2010. Slu. Available from: http://stud.epsilon.slu.se/4904/.
Baker J, Hardy MP, Zhou J, Bondy C, Lupu F, et al. Effects on an IGF I gene null mutation on mouse reproduction. Mol. Endocrinol: 1996,10903-918.
Doupont J, LeRoith D.Insulin and insulin like growth factor I receptor: similarities and differences in signal trandunction. Horm.Res. 2001,55: 22-26. doi.org/10.1159/000063469
Andersson L, Haley C, Ellegren H, Knott SA, Johansson M, et al.Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science.1998, 263: 1771–1774. doi.org/10.1126/science.8134840
Akhtar A, Shady Y, Ola W, Rajesh G, Leif A, et al. Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells.2015, Pnas: 1509193112. doi.org/10.1073/pnas.1509193112
Wood AW, Duan C and Bern HA. Insulin-like growth factor signaling in fish. Int Rev Cytol. 2005,243: 215-285.doi.org/10.1016/S0074-7696(05)43004-1
Markljung E, Jiang L, Jaffe JD, Mikkelsen TS, Wallerman O. ZBED6 a novel transcription factor derived from domesticated DNA transposon regulates IGF2 expression and muscle growth.2009, Plos Bio. 712: 100-0256.doi.org/10.1371/journal.pbio.1000256
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 MARKHOR (The Journal of Zoology)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open-access journal and all the published articles / items are distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. For comments editor@markhorjournal.com