Acute Toxicity and Behavioral Anomalies in Labeo Rohita Due to Exposure of Expired Permethrin, Malathion and Dichlorovos Mixture
Acute Toxicity in Labeo Rohita Due to Expired Permethrin, Malathion and Dichlorovos
DOI:
https://doi.org/10.54393/mjz.v6i3.182Keywords:
Anomalies, Acute Toxicity, Expired Pesticide Mixture, Labeo RohitaAbstract
greater threat to freshwater organisms. Objectives: To measure the tolerance limit of Labeo rohita to expired permethrin, malathion, and dichlorovos mixture at different concentrations. Methods: Freshwater fingerlings of L. rohita were exposed to sub-lethal (0.3mg/l) and lethal (2.3mg/l) concentrations of permethrin, malathion, and dichlorovos mixture, with three replications under constant water temperature (16-30°C), total water hardness (225mgL-1) and pH (6.8-7.7) for 96-hr. Probit analysis was used to calculate the Lethal concentration (LC50) and (LC100) of 96-hour exposure of the pesticide mixture for fish. Correlation analyses were conducted to identify relationships between the variables under study. Physicochemical parameters of water (Electrical conductivity (EC), Dissolved oxygen (DO), Oxidation-reduction potential (ORP), and Salinity) were recorded on a 12-hr basis. Results: For 96 hours, the mean values of LC50 and LC100 of L. rohita were calculated as 1.53 ± 0.028 and 3.05 ± 0.083 mgL-1, respectively. L. rohita exhibited behavioural changes such as erratic swimming, loss of equilibrium, heavy breathing, and abnormal posture due to the toxic effects of the pesticide mixture. Correlation analysis indicates that increasing concentrations of permethrin, malathion, and dichlorvos pesticides significantly degrade water quality, reducing DO while increasing ORP, salinity, and EC. Conclusions: It was concluded that an expired pesticide mixture is more lethal to aquatic life than an unexpired pesticide mixture. So, it is important to take precautionary measures before discarding expired pesticides in aquatic ecosystems.
References
Mishra A and Devi Y. Histopathological Alterations in the Brain (Optic Tectum) of the Freshwater Teleost Channa Punctatus in Response to Acute and Subchronic Exposure to the Pesticide Chlorpyrifos. Acta Histochemica. 2014 Jan; 116(1): 176-81. doi: 10.1016/j.acthis.2013.07.001.
Sabra FS and Mehana ES. Pesticide toxicity in fish with particular reference to insecticides. Asian Journal of Agriculture and Food Sciences. 2015 Feb; 3(1).
Pandey S, Kumar R, Sharma S, Nagpure NS, Srivastava SK, Verma MS. Acute Toxicity Bioassays of Mercuric Chloride and Malathion on Air-Breathing Fish Channa Punctatus (Bloch). Ecotoxicology and environmental safety. 2005 May; 61(1): 114-20. doi: 10.1016/j.ecoenv.2004.08.004.
Somaiah K, Satish PV, Sunita K, Jyothi NB. Studies on the Biochemical Responses in the Tissues of Freshwater Fish Labeo Rohita Exposed to The Organophosphate, Phenthoate. Environment. 2015; 5: 6.
Zhu Q, Yang Y, Lao Z, Zhong Y, Zhang K, Zhao S. Photo-degradation Kinetics, Mechanism and Aquatic Toxicity of Deltamethrin, Permethrin and Dihaloacetylated Heterocyclic Pyrethroids. Science of the Total Environment. 2020 Dec; 749: 142106. doi: 10.1016/j.scitotenv.2020.142106.
Wongmaneepratip W, Gao X, Yang H. Effect of Food Processing on Reduction and Degradation Pathway of Pyrethroid Pesticides in Mackerel Fillet (Scomberomorus Commerson). Food Chemistry. 2022 Aug; 384: 132523. doi: 10.1016/j.foodchem.2022.132523.
Van Cong N, Phuong NT, Bayley M. Brain Cholinesterase Response in the Snakehead Fish (Channa Striata) after Field Exposure to Diazinon. Ecotoxicology and Environmental Safety. 2008 Oct; 71(2): 314-8. doi: 10.1016/j.ecoenv.2008.04.005.
Ramakrishna R, Shipton TA, Hasan MR. Feeding and Feed Management of Indian Major Carps in Andhra Pradesh, India. FAO Fisheries & Aquaculture Technical Paper. 2013 Dec; (578).
Sharma A, Shukla A, Attri K, Kumar M, Kumar P, Suttee A et al. Global Trends in Pesticides: A Looming Threat and Viable Alternatives. Ecotoxicology and Environmental Safety. 2020 Sep; 201: 110812. doi: 10.1016/j.ecoenv.2020.110812.
Barbieri E and Ferreira LA. Effects of The Organophosphate Pesticide Folidol 600® On The Freshwater Fish, Nile Tilapia (Oreochromis Niloticus). Pesticide Biochemistry and Physiology. 2011 Mar; 99(3): 209-14. doi: 10.1016/j.pestbp.2010.09.002.
Murthy KS, Kiran BR, Venkateshwarlu M. A Review on Toxicity of Pesticides in Fish. International Journal of Open Scientific Research. 2013 May; 1(1): 15-36.
Aktar MW, Sengupta D, Chowdhury A. Impact of Pesticides Use in Agriculture: Their Benefits and Hazards. Interdisciplinary Toxicology. 2009 Mar; 2(1): 1. doi: 10.2478/v10102-009-0001-7.
Gill RJ and Raine NE. Chronic Impairment of Bumblebee Natural Foraging Behaviour Induced by Sublethal Pesticide Exposure. Functional Ecology. 2014 Dec; 28(6): 1459-71. doi: 10.1111/1365-2435.12292.
Mohan K, Sreenikethanam A, Raj S, Bajhaiya AK. Bioremediation and Phytoremediation of Environmental Pollutants: Advances and Current Strategies. In Biotechnology for Environmental Sustainability 2025 Feb (pp. 127-160). Singapore: Springer Nature Singapore. doi: 10.1007/978-981-97-7221-6_5.
Khwaja MA, Jan MR, Gul K. Physical Verification and Study of Contamination in and Around an Abandoned DDT Factory in North West Frontier Province (NWFP) Pakistan. International POPs Elimination Project (IPEP). 2006 May: 1-48.
Kreisler E and Heiss RO. Managing expired pesticides as hazardous waste across borders. In the Eighth International Conference on Environmental Compliance and Enforcement, Washington. 2008 Apr.
American Public Health Association, American Water Works Association, Water Pollution Control Federation, Water Environment Federation. Standard methods for the examination of water and wastewater. 3rd ed. American Public Health Association. 1917.
Helfrich LA, Weigmann DL, Hipkins PA, Stinson ER. Pesticides and Aquatic Animals: A Guide to Reducing Impacts On Aquatic Systems. 2009; May.
Satyavani G, Gopi RA, Ayyappan S, Balakrishnamurthy P, Reddy PN. Toxicity Effect of Expired Pesticides to Freshwater Fish, Labeo Rohita. Journal of Agriculture and Environment. 2011; 12: 1-9. doi: 10.3126/aej.v12i0.7557.
Kunwar PS, Parajuli K, Badu S, Sapkota B, Sinha AK, De Boeck G et al. Mixed Toxicity of Chlorpyrifos and Dichlorvos Show Antagonistic Effects in The Endangered Fish Species Golden Mahseer (Tor Putitora). Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology. 2021 Feb; 240: 108923. doi: 10.1016/j.cbpc.2020.108923.
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