Early Passage Characterization of Canine Synovial Fluid-Derived Stem Cells Isolated from Stifle Joint: Stem Cells Isolated from Stifle Joint

Muhammad Umar Sharif; Hafiz Muhammad Aslam; Tahira Iftakhar; Razia Kausar; Muhammad Abdullah

doi:10.54393/mjz.v6i1.155

Authors

Muhammad Umar Sharif Department of Anatomy, University of Agriculture, Faisalabad, Pakistan
Hafiz Muhammad Aslam Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan
Tahira Iftakhar College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China
Razia Kausar Department of Anatomy, University of Agriculture, Faisalabad, Pakistan
Muhammad Abdullah Department of Anatomy, University of Agriculture, Faisalabad, Pakistan

DOI:

https://doi.org/10.54393/mjz.v6i1.155

Keywords:

Synovial Stem Cells, Mesenchymal Markers, Bi-lineage Differentiation, Regenerative Medicine

Abstract

Synovial Fluid-Derived Stem Cells (SFSCs) have emerged as a promising source of mesenchymal stem cells, offering a minimally invasive means of obtaining cells with high proliferative capacity and robust multilineage differentiation potential. Originating from the synovial membrane, SFSCs are believed to retain a cellular bias towards musculoskeletal tissue repair, positioning them as a valuable tool in treating musculoskeletal injuries and diseases. Objective: To portray SFSCs differentiation behavior at early passage (P2) by evaluating their growth dynamics, immunophenotypic profile, and ability to differentiate into multilineages. Methods: In this experimental study, a typical MSC-like proliferation pattern was seen with distinct phases of lag, exponential and plateau growth curve. Immunohistochemistry revealed CD73⁺, CD90⁺, and CD105⁺ lacking hematopoietic markers, further validated their MSC like nature. Result: SFSC showed Bi-lineage differentiation into adipocytes and osteocytes validated by Oil O Red and Alizarin Red S staining respectively. Conclusions: In conclusion SFSC’s possesses regenerative potential, which could be a future of regenerative medicine to repair bones and soft tissues. These findings contribute to MSC biology and reinforce the therapeutic promise of SFSCs in musculoskeletal disorders.

References

Furuoka H, Endo K, Sekiya I. Mesenchymal stem cells in synovial fluid increase in number in response to synovitis and display more tissue-reparative phenotypes in osteoarthritis. Stem Cell Research & Therapy. 2023 Sep; 14(1): 244. doi: 10.1186/s13287-023-03487-1.

Harrell CR, Markovic BS, Fellabaum C, Arsenijevic A, Volarevic V. Mesenchymal stem cell-based therapy of osteoarthritis: Current knowledge and future perspectives. Biomedicine & Pharmacotherapy. 2019 Jan; 109: 2318-26. doi: 10.1016/j.biopha.2018.11.099.

Fellows CR, Matta C, Zakany R, Khan IM, Mobasheri A. Adipose, bone marrow and synovial joint-derived mesenchymal stem cells for cartilage repair. Frontiers in Genetics. 2016 Dec; 7: 213. doi: 10.3389/fgene.2016.00213.

Zheng YL, Sun YP, Zhang H, Liu WJ, Jiang R, Li WY et al. Mesenchymal stem cells obtained from synovial fluid mesenchymal stem cell-derived induced pluripotent stem cells on a matrigel coating exhibited enhanced proliferation and differentiation potential. PLOS One. 2015 Dec; 10(12): e0144226. doi: 10.1371/journal.pone.0144226.

Chen Y, Bianchessi M, Pondenis H, Stewart M. Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells. Stem Cell Biology and Research. 2016 Mar; 3(1). doi: 10.7243/2054-717X-3-1.

Neybecker P, Henrionnet C, Pape E, Grossin L, Mainard D, Galois L et al. Respective stemness and chondrogenic potential of mesenchymal stem cells isolated from human bone marrow, synovial membrane, and synovial fluid. Stem Cell Research & Therapy. 2020 Dec; 11: 1-2. doi: 10.1186/s13287-020-01786-5.

Kiefer KM, O'Brien TD, Pluhar EG, Conzemius M. Canine adipose‐derived stromal cell viability following exposure to synovial fluid from osteoarthritic joints. Veterinary Record Open. 2015 Jan; 2(1): e000063. doi: 10.1136/vetreco-2014-000063.

Rosa G, Krieck AM, Padula E, Pfeifer JP, de Souza JB, Rossi M et al. Allogeneic synovial membrane-derived mesenchymal stem cells do not significantly affect initial inflammatory parameters in a LPS-induced acute synovitis model. Research in Veterinary Science. 2020 Oct; 132: 485-91. doi: 10.1016/j.rvsc.2020.08.001.

Mocchi M, Bari E, Dotti S, Villa R, Berni P, Conti V et al. Canine mesenchymal cell lyosecretome production and safety evaluation after allogenic intraarticular injection in osteoarthritic dogs. Animals. 2021 Nov; 11(11): 3271. doi: 10.3390/ani11113271.

Ekkapol AK. The cellular physiology of canine chondrocytes: An in-vitro study on phenotype regulation and characteristics of cell death. (No Title). 2020 Apr;82(6): 793-803. doi: 10.1292/jvms.20-0118.

Francuski J, Debeljak-Martačić J, Radovanović A, Andrić N, Sourice-Petit S, Guicheux J et al. Proliferation and differentiation potential of canine synovial fluid cells. Acta Veterinaria-Beograd. 2015; 65(1): 66-78. doi: 10.1515/acve-2015-0005.

Tareen WA, Saba E, Rashid U, Sarfraz A, Yousaf MS, Rehman HF et al. Impact of multiple isolation procedures on the differentiation potential of adipose derived canine mesenchymal stem cells. American Journal of Stem Cells. 2024 Feb; 13(1): 27. doi: 10.62347/LEVZ7282.

Csaki C, Matis U, Mobasheri A, Ye H, Shakibaei M. Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochemistry and Cell Biology. 2007 Dec; 128: 507-20. doi: 10.1007/s00418-007-0337-z.

Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB. In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study. Stem Cell Research & Therapy. 2017 Dec; 8: 1-22. doi: 10.1186/s13287-017-0639-6.

Rashid U, Saba E, Yousaf A, Tareen WA, Sarfraz A, Rhee MH et al. Autologous platelet lysate is an alternative to fetal bovine serum for canine adipose-derived mesenchymal stem cell culture and differentiation. Animals. 2023 Aug; 13(16): 2655. doi: 10.3390/ani13162655.

Kim HR, Lee J, Byeon JS, Gu NY, Lee J, Cho IS et al. Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells. Journal of Veterinary Science. 2017 Sep; 18(3): 299-306. doi: 10.4142/jvs.2017.18.3.299.

Takemitsu H, Zhao D, Yamamoto I, Harada Y, Michishita M, Arai T. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells. BioMed Central Veterinary Research. 2012 Dec; 8: 1-9. doi: 10.1186/1746-6148-8-150.

Nantavisai S, Pisitkun T, Osathanon T, Pavasant P, Kalpravidh C, Dhitavat S et al. Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp. Scientific Reports. 2020 Nov; 10(1): 20703. doi: 10.1038/s41598-020-77656-0.

Madeira A, da Silva CL, Dos Santos F, Camafeita E, Cabral JM, Sá-Correia I. Human mesenchymal stem cell expression program upon extended ex-vivo cultivation, as revealed by 2-DE-based quantitative proteomics. PLoS One. 2012 Aug; 7(8): e43523. doi: 10.1371/journal.pone.0043523.

Garcia J, Wright K, Roberts S, Kuiper JH, Mangham C, Richardson J et al. Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: the influence of tissue source and inflammatory stimulus. Scientific Reports. 2016 Apr; 6(1): 24295. doi: 10.1038/srep24295.

Walczak BE, Jiao H, Lee MS, Li WJ. Reprogrammed synovial fluid-derived mesenchymal stem/stromal cells acquire enhanced therapeutic potential for articular cartilage repair. Cartilage. 2021 Dec; 13(2_suppl): 530S-43S. doi: 10.1177/19476035211040858.

Krawetz RJ, Affan A, Leonard C, Veeramreddy DN, Fichadiya A, Martin L et al. Synovial fluid mesenchymal progenitor cells from patients with juvenile idiopathic arthritis demonstrate limited self-renewal and chondrogenesis. Scientific Reports. 2022 Oct; 12(1): 16530. doi: 10.1038/s41598-022-20880-7.

Li F, Chen J, Gong M, Bi Y, Hu C, Zhang Y et al. Isolation and Characterization of Human Synovial Fluid‐Derived Mesenchymal Stromal Cells from Popliteal Cyst. Stem Cells International. 2020; 2020(1): 7416493. doi: 10.1155/2020/7416493.

Zayed M, Adair S, Dhar M. Effects of normal synovial fluid and interferon gamma on chondrogenic capability and immunomodulatory potential respectively on equine mesenchymal stem cells. International Journal of Molecular Sciences. 2021 Jun; 22(12): 6391. doi: 10.3390/ijms22126391.

Arévalo-Turrubiarte M, Olmeo C, Accornero P, Baratta M, Martignani E. Analysis of mesenchymal cells (MSCs) from bone marrow, synovial fluid and mesenteric, neck and tail adipose tissue sources from equines. Stem Cell Research. 2019 May; 37: 101442. doi: 10.1016/j.scr.2019.101442.

Jorgenson KD, Hart DA, Krawetz R, Sen A. Production of adult human synovial fluid‐derived mesenchymal stem cells in stirred‐suspension culture. Stem Cells International. 2018 Mar; 2018(1): 8431053. doi: 10.1155/2018/8431053.